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Automated calculation of morpho-histological parameters

DISCOVERIES (ISSN 2359–7232), 2016, July-September issue


Pop-Fele L, Curaj A, Jovanovici M, Jonas SM, Moellmann J, Ghertescu D, Novac OC, Rusu M. Liehn EA. Advanced modular automated calculation of the morpho-histological parameters in myocardial infarction. Discoveries 2016, Jul-Sep; 4(3): e66. DOI: 10.15190/d.2016.13

Submitted: September 28, 2016; Revised: September 30, 2016; Accepted: September 30, 2016; Published: September 30, 2016;

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Advanced modular automated calculation of the morpho-histological parameters in myocardial infarction

Lucian Pop-Fele (1,2,3), Adelina Curaj (1,4), Marko Jovanovic (2), Stephan Michael Jonas (2), Julia Moellmann (5), Doina Ghertescu (6), Ovidiu Constantin Novac (3), Mihaela Rusu (1,4), Elisa A. Liehn (1,4,5,7, *)

(1) Institute for Molecular Cardiovascular Research, University Hospital, RWTH Aachen, Germany; (2) Department of Medical Informatics, RWTH Aachen, Germany;  (3) Faculty of Electrical Engineering and Information Technology, University of Oradea, Oradea, Romania;  (4) IZKF, Aachen, RWTH Aachen, Germany;  (5) Department of Cardiology and Pulmonology, Medical Faculty, RWTH Aachen University, Germany;  (6) Cardiology Department, University of Medicine and Pharmacy, Targu Mures, Romania;  (7) Human Genetic Laboratory, University of Medicine and Pharmacy, Craiova, Romania;

*Correspondence to: Elisa A. Liehn, MD, PhD, Institute for Molecular Cardiovascular Research (IMCAR), University Hospital Aachen, Rheinisch-Westfälische Technische Hochschule Aachen, Pauwelsstrasse 30, 52074 Aachen, Germany; Tel.: 0049-241-80 35983; Fax: 0049-241-80 82716; Email: eliehn@ukaachen.de


     Myocardial infarction represents the most investigated pathology. Heart tissues are histologically assayed on a routine base in clinical laboratories. However, the lack of a standard operation procedure for e.g. calculating the size of the infarcted area of myocardium, may lead to an increased errors’ interval, with little correlation between results of a same tissue and/or with other pathophysiological parameters. This creates a clear barrier for further development of novel therapeutic strategies. In the present study, we present the robust applicability of a novel methodology such as:  advanced modular automated calculation of (i) the size of infarcted heart of mice, (ii) the net collagen content present in the scar, and (iii) the interstitial fibrosis in remote, which are simultaneously performed. The new approach of defining the infarct size, one of the important predictor of every cardiac therapeutic intervention, will create a positive impact in the research accuracy. Additionally, it will be expected that the readily assembled reports of simultaneously computed parameters and its user-friendly operation allow an efficient and effective estimation of measurements.

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1. Liehn EA, Postea O, Curaj A, Marx N. Repair after myocardial infarction, between fantasy and reality: The role of chemokines. J. Am. Coll. Cardiol. 2010;58:2357-2362.

2. Liehn EA, Radu E, Schuh A. Chemokine contribution in stem cell engraftment into the infarcted myocardium. Curr. Stem Cell Res. Ther. 2013;8:278-283.

3. Schuh A, Butzbach B, Curaj A, Simsekyilmaz S, Bucur O, Kanzler I, Denecke B, Konschalla S, Kroh A, Sönmez TT, Marx N, Liehn EA. Novel insights into the mechanism of cell-based therapy after chronic myocardial infarction. Discoveries 2014;1:e9.

4. Gibbons RJ, Valeti US, Araoz PA, Jaffe AS. The quantification of infarct size. J. Am. Coll. Cardiol. 2004;44:1533-1542.

5. Dewald O, Zymek P, Winkelmann K, Koerting A, Ren G, Abou-Khamis T, Michael LH, Rollins BJ, Entman ML, Frangogiannis NG. Ccl2/monocyte chemoattractant protein-1 regulates inflammatory responses critical to healing myocardial infarcts. Circ. Res. 2005;96:881-889.

6. Liehn EA, Tuchscheerer N, Kanzler I, Drechsler M, Fraemohs L, Schuh A, Koenen RR, Zander S, Soehnlein O, Hristov M, Grigorescu G, Urs AO, Leabu M, Bucur I, Merx MW, Zernecke A, Ehling J, Gremse F, Lammers T, Kiessling F, Bernhagen J, Schober A, Weber C. Double-edged role of the cxcl12/cxcr4 axis in experimental myocardial infarction. J. Am. Coll. Cardiol.  2010;58:2415-2423.

7. Takagawa J, Zhang Y, Wong ML, Sievers RE, Kapasi NK, Wang Y, Yeghiazarians Y, Lee RJ, Grossman W, Springer ML. Myocardial infarct size measurement in the mouse chronic infarction model: Comparison of area- and length-based approaches. J. Appl. Physiol.  2007;102:2104-2111.

8. Curaj A, Simsekyilmaz S, Staudt M, Liehn E. Minimal invasive surgical procedure of inducing myocardial infarction in mice. J Vis Exp. 2015; e52197.

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